Temporary

Part:BBa_J133002:Design

Designed by: Norman Wang   Group: Presting Lab   (2010-12-17)


GFPFrag+OligoN


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 829
    Illegal NotI site found at 21
    Illegal NotI site found at 835
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 900
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 849


Design Notes

gene synthesis from IDTDNA, $220 (max 400bp), ordered on 9/22/10, arrived 10/27/10


Source

Source DNA: backbone: PCRed using G1000-G1001 from pSB3K5-J732720 insert1: PCRed using custom mut primers g0001_f-g0002_r from E0240 E Coli cryostock insert2: insert freed from pIDTSMART-OligoN plasmid prep

References